Entrants must create a new work of between 40 and 60 seconds length, which follows the theme 'Underdog'.

Production techniques used are completely open and could include stop-frame, traditional 2D, motion graphics, 3D, photography, puppetry, film or video. The result must ensure a compelling experience for the viewer rather than just a display of technical prowess.

Ten finalists will be screened and the winner announced at the opening night of the UTS: Sydney International Animation Festival, Friday 24 September, 2010. As well, the ten finalists will have their work streamed from the Proto-Ninja award website for a period of 12 months or until the 2011 winner is announced.