Mary Ainslee (October 12, 1919 – November 1, 1991) was an American film actress. She appeared in approximately 15 films between 1939 and 1952.

Ainslee appeared in several Three Stooges films such as I'll Never Heil Again, In the Sweet Pie and Pie, Hokus Pokus, and He Cooked His Goose.

In the mid-1980s, she suffered a stroke and never fully recovered. She died on November 1, 1991.