Chloe Ashcroft (born 7 August 1942) is a former presenter of several BBC children's TV programmes, including Play School, Play Away, Hokey-Cokey and Pie in the Sky. She also appeared in the Doctor Who story Resurrection of the Daleks, playing Professor Laird.

Outside of acting, she has worked as a teacher. She now lives in Whiteshill and Ruscombe in Gloucestershire with her husband, the actor David Hargreaves.